FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830883

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830883
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11497430
Sequences flagged as poor quality0
Sequence length50-126
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC109100.079.24207120
GAGCACA182600.045.76865120
AGAGCAC250450.035.241035120
AAGAGCA412750.021.140326118-119
GAAGAGC433200.020.79161120
GGAAGAG513850.017.7238120
CGGAAGA567700.016.148748120
TCGGAAG595100.015.3995905118-119
ATCGGAA702200.013.904361120
GATCGGA732500.012.92695118-119
CACGTCT31950.012.78537120
CACACGT27550.012.153538120
AGATCGG820750.011.79809120
TAGATCG209950.011.004177118-119
AAGATCG371400.09.637405118-119
CTAGATC58050.08.6519165116-117
ACACGTC36200.08.601985118-119
CAGATCG234200.08.506547116-117
TAAGATC143250.08.414575112-113
GAGATCG251900.08.413949118-119
Produced by FastQC (version 0.11.5)