FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830884

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830884
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14961243
Sequences flagged as poor quality0
Sequence length52-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG94400.013.8549120
AAAAAAA1805500.013.089027120
GTATCAT725050.08.729782120
ATCATTA674000.08.62547120
CCGTATC829950.08.501084120
TGGTCGC1181750.08.47832120
TCATTAA602050.08.430567120
CGTATCA727650.08.368786120
GCCGTAT879150.08.291483120
GTCGCCG1094850.08.192294120
GGTCGCC1120850.08.10396120
CATTAAA600700.08.030033120
CGCCGTA943050.08.009584120
TCGCCGT1034900.07.739302120
CGGTGGT1471100.07.6875525120
GGTGGTC1357950.07.630052120
TATCATT762550.07.5923796120
GTGGTCG1262800.07.577833120
TCGGTGG1554950.07.373321120
TCTCGGG40850.07.049659120
Produced by FastQC (version 0.11.5)