FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830885

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830885
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14961243
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC207000.0101.74077120
GAGCACA347050.060.753067120
AGAGCAC474800.044.573742118-119
AAGAGCA703050.030.028566118-119
GAAGAGC804800.026.286854116-117
GGAAGAG989400.021.309502116-117
CGGAAGA1163150.018.282059114-115
TCGGAAG1176300.017.887136114-115
ATCGGAA1370400.015.621014112-113
GATCGGA1419700.014.934771112-113
CACACGT45900.014.649106120
AGATCGG1541000.013.889089110-111
CACGTCT53250.013.679377120
TAGATCG352050.012.65482112-113
ACACGTC53850.012.262997118-119
CTAGATC66800.011.445193110-111
GAGATCG648400.011.16765110-111
ACGTCTG65450.010.884911120
CAGATCG526500.010.430879112-113
AAGATCG429450.010.262211110-111
Produced by FastQC (version 0.11.5)