FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830889

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830889
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15824742
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC204450.084.11253120
GAGCACA329050.050.67169120
AGAGCAC447400.038.004738118-119
AAGAGCA686250.024.693655118-119
GAAGAGC762250.022.315561116-117
GGAAGAG917750.018.452555116-117
CGGAAGA1077950.016.15275120
TCGGAAG1078600.015.641402118-119
ATCGGAA1267250.013.515802112-113
GATCGGA1269100.013.321397116-117
AGATCGG1387900.012.23251110-111
TAGATCG279050.011.472508114-115
CTAGATC67600.010.497989120
AAGATCG635700.010.100224114-115
GAGATCG449250.09.446072120
TTAGATC121100.09.3146715108-109
CAGATCG399100.09.178184120
TAAGATC263100.08.670064110-111
CACGTCT55050.08.333745120
TGAGATC222950.08.271816108-109
Produced by FastQC (version 0.11.5)