FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830899

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830899
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12442476
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC141100.073.71418120
GAGCACA224800.044.304317120
AGAGCAC299650.035.46968120
AAGAGCA454600.023.394585120
GAAGAGC525550.020.949005120
GGAAGAG640700.017.105362118-119
TCGGAAG750750.014.780826120
CGGAAGA756800.014.583121120
ATCGGAA882000.012.603844118-119
GATCGGA887050.012.487052120
AGATCGG989200.011.285477120
TAGATCG244750.010.781119116-117
CCTAGAT22700.010.607775120
CTAGATC60100.010.127599116-117
TTAGATC82000.08.932462108-109
AAGATCG333800.08.616478120
GAGATCG419500.08.323276114-115
ATAGATC128400.07.9702888120
GCTAGAT23100.07.9629955114-115
TCTAGAT30250.07.960215120
Produced by FastQC (version 0.11.5)