FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830907

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830907
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13600105
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC120700.059.22077120
GAGCACA190400.037.980236120
AGAGCAC242400.031.10439120
AAGAGCA416900.018.100567120
GAAGAGC420400.018.08736120
GGAAGAG527800.015.538452120
TCGGAAG582050.014.3660345120
CGGAAGA603550.013.238389118-119
AACCCTA24850.012.18491986-87
GATCGGA718400.011.657287120
ATCGGAA707100.011.434867120
TAGATCG183800.011.390906120
AGATCGG774350.010.570846118-119
GAGTAGG31850.010.4951684
TGAGTAG31100.010.3711363
GTGAGTA32700.010.0430192
AGTGAGT30400.010.039011
ACCCTAT26100.09.39709386-87
CTAGATC50500.09.2836275120
TTAACCC34000.09.22106784-85
Produced by FastQC (version 0.11.5)