FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830911

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830911
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16011284
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC193450.085.225845120
GAGCACA311500.051.80826120
AGAGCAC424600.038.55344118-119
AAGAGCA655250.025.269892120
GAAGAGC739200.022.41118-119
GGAAGAG922350.018.422668120
TCGGAAG1074050.015.486311118-119
CGGAAGA1080850.015.471174116-117
ATCGGAA1259800.013.246015114-115
GATCGGA1293450.012.8869705116-117
AGATCGG1392750.012.021614120
TAGATCG347300.011.791922114-115
CTAGATC74550.010.547638112-113
GAGATCG585700.09.937842120
TTAGATC120000.09.220829116-117
CCTAGAT26250.09.081469112-113
AAGATCG426550.08.908245116-117
CAGATCG451900.08.432593114-115
CTTCCGA133450.07.94054841
CGATCTA21400.07.89184575
Produced by FastQC (version 0.11.5)