FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830913

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830913
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14724033
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC131450.062.047466120
GAGCACA200050.038.436195120
AGAGCAC260050.030.800173118-119
AAGAGCA420750.021.011566120
GAAGAGC460600.019.415909120
GGAAGAG568950.016.482927120
TCGGAAG651950.014.6494055120
CGGAAGA678150.014.140031120
ATCGGAA783000.012.393624120
GATCGGA791100.012.200326118-119
AGATCGG877050.011.261531120
TAGATCG240200.010.626111120
CTAGATC66650.09.089983114-115
GAGATCG334200.08.134989120
TTAGATC105900.08.009122116-117
AAGATCG365900.07.9022455120
CAGATCG333050.06.9410915116-117
CCCTAGA10350.00248912426.798715120
CCTAGAT25950.06.7580986116-117
ATAGATC137300.06.2934556118-119
Produced by FastQC (version 0.11.5)