FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830914

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830914
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13649481
Sequences flagged as poor quality0
Sequence length53-126
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG65500.010.8994875120
TAGATCT382600.010.897836120
AGATCTC365650.010.664688120
ATCTCGG319350.010.012918120
GATCTCG345900.09.469836120
CTCGGTG275200.09.461102120
AGTGTAG548250.09.35595120
GAGTGTA591800.09.113499120
GTGGTCG188350.09.1096325120
TCGGTGG265700.09.076845120
TCTCGGT289150.09.0254120
AGAGTGT646900.08.902962120
GTGTAGA527900.08.898372120
GTAGATC519700.08.519302120
GGTGGTC218050.08.474129120
TGGTCGC169350.08.43127118-119
CGGTGGT261250.08.35883120
GGTCGCC175900.08.356043120
AAGAGTG721350.08.133795120
TGTAGAT568100.08.004696120
Produced by FastQC (version 0.11.5)