FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830915

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830915
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13649481
Sequences flagged as poor quality0
Sequence length50-126
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC142950.072.28172120
GAGCACA222250.049.126705120
AGAGCAC308500.035.285263120
AAGAGCA491300.022.592825118-119
GAAGAGC541400.021.686506120
GGAAGAG668900.017.120604118-119
CGGAAGA812650.014.335099118-119
TCGGAAG813800.014.303183116-117
ATCGGAA958650.012.714342120
GATCGGA953950.012.204871118-119
AGATCGG1053450.011.273894118-119
TAGATCG265400.010.886207120
CTAGATC69600.010.070971118-119
AAGATCG404050.08.967422116-117
GAGATCG380450.08.926538118-119
CACGTCT49800.08.854386120
TTAGATC110450.08.759856116-117
ATAGATC122150.08.4590025120
ACACGTC51750.08.393567120
CAGATCG294550.08.200727116-117
Produced by FastQC (version 0.11.5)