FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830917

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830917
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1711568
Sequences flagged as poor quality0
Sequence length50-126
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC14900.044.63324120
GAGCACA20450.026.79894120
AGAGCAC24050.024.579727118-119
AAGAGCA38900.015.354761120
GAAGAGC42150.014.243869116-117
TCGGAAG48250.013.910736120
TAGGGGC3050.001222044113.6659763
GTTAGGG2650.00762069513.4817988
GGAAGAG48500.011.998059118-119
GATCGGA56200.011.997721118-119
TAGATCG14700.011.310123118-119
ATCGGAA60650.011.16819120
TTAGATC6351.7906132E-810.666942120
CGGAAGA55150.010.662985114-115
AGATCGG65250.09.720254118-119
CTAGATC4651.4799585E-59.269708120
GACACGT3952.3731691E-48.4733398-99
AAGATCG30400.07.292053116-117
GAGATCG33600.06.5059395116-117
ATAGATC9251.3936569E-66.3241596116-117
Produced by FastQC (version 0.11.5)