FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830922

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830922
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16388881
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCT75400.035.250624
TCCGATC80250.033.568593
CGATCTA20200.031.483935
CTTCCGA136350.020.2929211
TTCCGAT174750.015.7589492
AAAAAAA2071850.014.318606120
CGATCTG86700.010.9338325
CGTATCA974700.09.820859120
GATCTAA19850.09.672176
CATTAAA719500.09.615099120
CGATCTC79000.09.569255
ATCATTA836500.09.53376120
TCATTAA744400.09.470956120
GCGTCGG116000.09.370282120
CGCCGTA1263000.09.07684120
TCTCGGG47700.09.0645485120
CCGTATC1076000.08.963246120
GTATCAT938500.08.900717120
GGTCGCC1526700.08.649752120
GCCGTAT1182100.08.554998120
Produced by FastQC (version 0.11.5)