FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830924

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830924
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13234819
Sequences flagged as poor quality0
Sequence length59-126
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGTAG283900.08.020098120
TAGATCT214450.08.012091120
GAGTGTA313450.07.359848120
GTGTAGA286200.07.283929120
AGAGTGT340100.07.171752120
GTAGATC298450.07.1661234120
AGATCTC222400.07.0233517120
GTAGGGA508600.06.9101167120
TGTAGGG543300.06.6788716120
AAGAGTG378050.06.6743026120
TAGGGAA490150.06.6222324118-119
GTGTAGG589900.06.517896120
ATCTCGG254450.06.4692507120
TGTAGAT308500.06.4653015120
CGTGTAG621650.06.41694120
GTCGTGT680600.06.3731055120
GGGAAAG518550.06.3256893120
TCGGTGG206600.06.2519283120
AGGGAAA553750.06.1514173120
GGTGGTC174050.06.074975120
Produced by FastQC (version 0.11.5)