FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830933

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830933
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16971584
Sequences flagged as poor quality0
Sequence length50-126
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC280450.0105.793175120
GAGCACA468350.060.86765120
AGAGCAC634800.046.046932120
AAGAGCA953450.030.598984118-119
GAAGAGC1041300.027.934053116-117
GGAAGAG1254400.023.099545116-117
CGGAAGA1449450.020.17182114-115
CACGTCT79900.019.39651120
TCGGAAG1520800.019.143541114-115
ACACGTC77100.018.382427118-119
CACACGT72050.017.346119118-119
ATCGGAA1785500.016.565287114-115
GATCGGA1830250.016.05598112-113
ACGTCTG99300.015.867177120
AGATCGG1984550.014.902829112-113
GCACACG91750.014.076123120
TAGATCG526500.013.670213110-111
CGTCTGA117350.012.986377120
CTAGATC114000.012.506416110-111
GAGATCG694650.012.419769110-111
Produced by FastQC (version 0.11.5)