FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830937

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830937
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14145268
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC149100.064.93627120
GAGCACA224000.040.310272120
AGAGCAC293750.032.196682118-119
AAGAGCA455150.022.387115120
GAAGAGC512200.019.430347120
GGAAGAG638550.016.473322120
TCGGAAG746050.014.391177120
CGGAAGA742150.013.933761118-119
ATCGGAA891500.012.531168120
GATCGGA896850.012.257996120
TAGATCG234150.011.625165120
AGATCGG1000200.011.617393120
CTAGATC59800.010.250026120
CACGTCT66750.09.923203118-119
CACACGT50750.09.350603120
CGTCTGA86450.09.301179120
GAGATCG408300.09.071812118-119
ACGTCTG74550.08.973369118-119
AAGATCG318100.08.287422116-117
CAGATCG378250.08.154739120
Produced by FastQC (version 0.11.5)