FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830940

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830940
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14763377
Sequences flagged as poor quality0
Sequence length50-126
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGGA227400.011.106618120
TAGGGAA212200.010.432087120
TCGTGTA353700.09.990114120
AGAGTGT113850.09.906381120
TGTAGGG252500.09.669928120
GAGTGTA99400.09.6565895120
CGTGTAG329700.09.570992120
AGTGTAG91950.09.264603120
AAGAGTG141000.09.232744120
GTCGTGT394050.09.104164120
GTGTAGG299850.08.903201120
GGGAAAG242500.08.78227120
AGGGAAA264550.08.481136120
AGAGCGT587650.08.40178120
CTAGATC67000.08.237648120
AAAGAGT175900.08.117108120
TCGGAAG865250.08.070521120
GAAGAGC726050.07.965274120
AGCGTCG570750.07.946321120
AAGAGCG639500.07.842525120
Produced by FastQC (version 0.11.5)