FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830941

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830941
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14763377
Sequences flagged as poor quality0
Sequence length50-126
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC122900.036.24948120
GAGCACA178300.026.704775120
AGAGCAC197650.026.291677120
AAGAGCA283750.018.767359120
GAAGAGC354150.016.957354120
GGAAGAG419100.015.045858120
TCGGAAG497650.014.801295120
CGGAAGA519350.012.873121120
GATCGGA631700.011.922306120
ATCGGAA611200.011.911114120
AGATCGG713700.010.921711120
CTAGATC43250.010.909978120
TAGATCG155900.010.495048120
TTAGATC57400.08.754296118-119
TCTAGAT23300.07.101124118-119
GAGATCG362300.06.6980147120
ATAGATC77500.06.641969120
AAGATCG366300.06.6081433120
CCTAGAT19200.06.2237573116-117
GTAGATC93150.06.0523524120
Produced by FastQC (version 0.11.5)