FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830950

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830950
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11945476
Sequences flagged as poor quality0
Sequence length54-126
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGTAG131800.010.924723120
AAGAGTG193350.010.332719120
TAGATCT97300.09.74224120
TAGGGAA291050.09.7294655120
AGAGTGT165600.09.709325120
GAGTGTA143500.09.699531120
TGTAGGG339500.09.383597120
GTAGGGA310400.08.91033120
GTGTAGA144950.08.774699120
GTGTAGG382650.08.591984120
GTCGTGT479450.08.546573120
TCGTGTA439450.08.423454120
GTAGATC164150.08.3331375120
GGGAAAG328250.08.060248120
AAAGAGT226950.08.049519118-119
AGCGTCG656200.07.999934120
CGTGTAG411350.07.861222118-119
AGGGAAA355500.07.7630577120
GAGCGTC712100.07.4224887120
TGTAGAT162300.07.31916120
Produced by FastQC (version 0.11.5)