FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830953

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830953
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12095111
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC160500.082.51978120
GAGCACA251850.048.438347120
AGAGCAC341750.038.391125120
AAGAGCA498050.025.65387118-119
GAAGAGC569550.022.57402120
GGAAGAG670500.019.039854116-117
CGGAAGA799850.016.253208116-117
TCGGAAG805400.015.900701114-115
ATCGGAA946200.013.731579114-115
GATCGGA965200.013.310604112-113
AGATCGG1065600.012.217701112-113
CACACGT40550.012.076995120
CTAGATC60500.011.839835120
TAGATCG246700.011.659914112-113
TTAGATC87700.010.668057120
CACGTCT43900.010.15641120
AAGATCG420800.09.501789114-115
GAGATCG366750.09.188275110-111
ACACGTC47250.08.972245120
ATAGATC112600.08.926558112-113
Produced by FastQC (version 0.11.5)