FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830955

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830955
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12225057
Sequences flagged as poor quality0
Sequence length50-126
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC163750.098.33711120
GAGCACA259950.060.361103120
AGAGCAC363200.044.063503120
AAGAGCA523100.030.468304120
GAAGAGC630200.025.577873120
GGAAGAG750950.021.56618118-119
CGGAAGA884500.018.272684118-119
TCGGAAG893100.018.036865116-117
ATCGGAA1022650.015.808298114-115
GATCGGA1065150.015.266434114-115
AGATCGG1170550.013.891444112-113
CACACGT46250.012.6394005118-119
TAGATCG215950.012.434159120
CTAGATC44750.012.4188385112-113
CACGTCT51350.012.030199120
ACACGTC52800.011.855823120
TTAGATC61200.011.299079108-109
GAGATCG491250.011.1526985110-111
AAGATCG390350.010.640872112-113
CCCTAGA5906.47251E-810.4646435108-109
Produced by FastQC (version 0.11.5)