FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830957

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830957
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13102378
Sequences flagged as poor quality0
Sequence length50-126
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC135500.058.137074120
GAGCACA204500.039.90476120
AGAGCAC261150.031.91327120
AAGAGCA400500.021.451632120
GAAGAGC472200.018.562063120
GGAAGAG562450.016.17815120
TCGGAAG649250.014.4411545120
CGGAAGA667600.013.408472120
GATCGGA759700.012.993394120
ATCGGAA757900.012.820618120
AGATCGG856950.011.698954120
TAGATCG211050.011.578561120
CTAGATC51450.010.436781118-119
GAGATCG340400.09.993612120
TTAGATC77150.09.835965116-117
CAGATCG320350.08.370989118-119
GCTAGAT20200.08.277102120
AAGATCG280500.08.242082116-117
CCCTAGA7258.1385224E-87.98307118-119
GCCTAGA9351.8062565E-97.5657496116-117
Produced by FastQC (version 0.11.5)