FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830963

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830963
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11221451
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC151500.062.901432120
GAGCACA221500.041.60188120
AGAGCAC287800.032.577007118-119
AAGAGCA449150.022.58861120
GAAGAGC485150.020.49834120
GGAAGAG596550.016.850086120
TCGGAAG685900.015.113966120
ACACGTC50450.014.468837120
CGGAAGA693850.014.376354118-119
CACACGT45750.012.80828118-119
ATCGGAA837300.012.629011120
CACGTCT49100.012.411612120
GATCGGA855850.012.359357118-119
AGATCGG958250.011.293558120
TAGATCG208250.010.290557118-119
CTAGATC52200.09.943116-117
TTAGATC79100.09.651541120
ACGTCTG66950.09.602603120
AAGATCG396550.08.697522116-117
CCTAGAT17600.08.561379118-119
Produced by FastQC (version 0.11.5)