FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830967

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830967
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12964495
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC147650.068.77366120
GAGCACA236900.046.35827120
AGAGCAC314300.034.4747120
AAGAGCA491050.022.514631118-119
GAAGAGC533800.020.523735120
GGAAGAG636100.017.642847120
CGGAAGA801650.014.973774120
TCGGAAG818800.014.684605120
CACACGT64700.013.104662120
GATCGGA988050.012.838109120
ATCGGAA967200.012.569558120
ACACGTC67300.012.003189120
AGATCGG1099150.011.2976118-119
CACGTCT63300.010.968819118-119
TAGATCG281400.010.794777120
CTAGATC72000.010.477854120
ACGTCTG71450.09.717651118-119
CGTCTGA85850.09.254075120
TTAGATC113700.08.778135114-115
GAGATCG416200.08.694079120
Produced by FastQC (version 0.11.5)