FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830969

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830969
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18645924
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC207750.083.084206120
GAGCACA348300.049.001915120
AGAGCAC460900.038.568924120
AAGAGCA702350.025.096113118-119
GAAGAGC812050.021.881973120
GGAAGAG990550.017.884796118-119
CGGAAGA1166500.015.404882120
TCGGAAG1170500.015.162763118-119
CCCTAGA12100.013.613976120
ATCGGAA1348900.013.242352116-117
GATCGGA1355300.013.1373625120
AGATCGG1482050.012.062136120
TAGATCG364400.011.399743112-113
CTAGATC85300.010.999291120
TTAGATC131600.09.741942116-117
GAGATCG512350.09.526811114-115
AAGATCG527600.09.5025835116-117
CAGATCG474200.09.182999110-111
TGAGATC215350.08.098406112-113
CCTAGAT30200.08.063331120
Produced by FastQC (version 0.11.5)