FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830972

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830972
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17353771
Sequences flagged as poor quality0
Sequence length53-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATCT154850.07.981326120
AGTGTAG211750.07.961629120
GAGTGTA229800.07.8845334120
GTAGATC218800.07.5679946120
CGTGTAG503900.07.38187120
TAGGGAA409500.07.2961626120
GTGTAGG477500.07.287418120
AGAGTGT253700.07.2836537120
GTAGGGA410250.07.282824120
TGTAGGG429900.07.159274120
AAGAGTG293650.07.1304092120
TCGTGTA538400.06.986852120
AGCGTCG675450.06.8749065120
AGATCTC174650.06.8360233120
GTCGTGT556100.06.759074118-119
GTGTAGA227800.06.7422566120
CGTCGTG623750.06.636783120
GCGTCGT648500.06.568518120
GAGCGTC741750.06.5030565120
AGAGCGT786100.06.449083120
Produced by FastQC (version 0.11.5)