FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830973

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830973
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17353771
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC104550.045.257435120
GAGCACA153300.032.18991120
AGAGCAC195750.027.03238120
AAGAGCA350850.015.450481120
GAAGAGC381300.014.11174118-119
TCGGAAG485550.011.823207120
GATCGGA545800.011.589032120
GGAAGAG492400.011.533769120
AGATCGG599450.010.705794120
ATCGGAA592550.010.602013120
TAGATCG152900.09.859303120
CGGAAGA577150.09.786819120
CTAGATC41000.08.704258120
AAGATCG308500.07.1801734120
GAGATCG247100.07.096753118-119
TTAGATC81100.07.09379118-119
CAGATCG241300.05.890365120
TAAGATC156900.05.451041120
CCCCCCC29650.05.21910944-45
ACCCTAG8250.00414645535.050310646-47
Produced by FastQC (version 0.11.5)