FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830977

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830977
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13082129
Sequences flagged as poor quality0
Sequence length50-126
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC204750.0153.7417120
GAGCACA340050.090.79716120
AGAGCAC455850.068.60497118-119
AAGAGCA678700.045.047962118-119
GAAGAGC751000.041.522743116-117
GGAAGAG897700.033.9042116-117
CGGAAGA1061450.029.492338114-115
TCGGAAG1099000.027.771309114-115
ATCGGAA1266700.024.92778112-113
GATCGGA1288100.023.909693112-113
AGATCGG1383350.022.821405110-111
CACACGT55550.022.145792120
ACACGTC59750.021.598368120
TAGATCG355550.020.70965110-111
CACGTCT62800.020.165302120
CTAGATC71600.019.708818110-111
TTAGATC124250.019.316648108-109
CAGATCG449700.018.881544110-111
GAGATCG451250.018.723139110-111
AAGATCG365650.018.109001110-111
Produced by FastQC (version 0.11.5)