FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830978

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830978
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15089863
Sequences flagged as poor quality0
Sequence length53-126
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC65400.039.4478343
CCGATCT79100.031.8570294
CGATCTA18150.026.77575
CTTCCGA131650.020.8307781
TTCCGAT148500.018.1406292
AAAAAAA2119250.014.857514120
CGATCTC80300.010.9086185
GCGTCGG120200.010.800136120
CGTATCA971450.09.99153120
TCATTAA723300.09.804907120
ATCATTA804150.09.603865120
CATTAAA686750.09.600369120
GATCTAG11102.8714567E-89.1887926
CCGTATC1083950.08.993351120
CGATCTG93000.08.9673545
GTATCAT918750.08.955421120
TATCATT915300.08.844719120
GCCGTAT1184050.08.776167120
TCTCGGG45250.08.766077120
CGCCGTA1268050.08.763558120
Produced by FastQC (version 0.11.5)