FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830980

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830980
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13671353
Sequences flagged as poor quality0
Sequence length69-126
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTGTA132500.010.700895120
TAGGGAA271500.09.780846120
GTAGGGA276400.09.281407120
AGAGTGT151400.09.206321120
TGTAGGG307450.09.106163120
AGTGTAG121250.08.96852120
GTGTAGG340950.08.8281765120
CGTGTAG364400.08.738191120
TAGATCT89000.08.640601120
AAGAGTG184650.08.492102120
TCGTGTA390350.08.064939120
GTAGATC145300.08.062932120
GTCGTGT414750.07.9381294120
GTGTAGA133000.07.6236234118-119
CGTCGTG492800.07.4733233120
AGCGTCG554050.07.460423120
AGAGCGT629550.07.224198120
AAGAGCG692200.07.1605496120
GAGCGTC600350.07.115232120
AGGGAAA373250.06.921379120
Produced by FastQC (version 0.11.5)