FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830981

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830981
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13671353
Sequences flagged as poor quality0
Sequence length50-126
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC102550.040.89281120
GAGCACA151700.028.578764120
AGAGCAC177600.026.21668120
AAGAGCA308150.016.390621120
GAAGAGC328200.015.314144120
GGAAGAG396800.013.148384120
TCGGAAG422400.012.993908120
GATCGGA505000.012.101968120
CGGAAGA456950.011.283307118-119
ATCGGAA519800.011.164186120
AGATCGG567200.010.542786118-119
TAGATCG155850.010.16951120
CTAGATC41600.08.72102116-117
TTAGATC73200.07.919367120
AAGATCG264600.06.992059120
CCTAGAT18450.06.832557114-115
GAGATCG266100.06.7904167120
CACGTCT42550.05.652476120
CACACGT37350.05.5974507118-119
ATAGATC93750.05.509556116-117
Produced by FastQC (version 0.11.5)