FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830983

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830983
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13688509
Sequences flagged as poor quality0
Sequence length50-126
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC166200.075.68473120
GAGCACA261750.049.086903120
AGAGCAC356500.036.23464120
AAGAGCA524000.024.916435118-119
GAAGAGC611850.021.801874120
GGAAGAG752300.018.007355120
TCGGAAG890450.015.508689120
CGGAAGA891100.015.132641120
ATCGGAA1036050.013.246027118-119
GATCGGA1073200.012.951556120
AGATCGG1195250.011.536701118-119
TAGATCG256200.011.174715118-119
CTAGATC59950.010.612971114-115
TTAGATC89100.09.818113118-119
TCTAGAT26850.08.885609116-117
CACGTCT53400.08.80591120
GAGATCG492600.08.781025116-117
ACTAGAT21750.08.584422120
CACACGT45150.08.577028120
AAGATCG424250.08.541313118-119
Produced by FastQC (version 0.11.5)