FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830984

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830984
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11523913
Sequences flagged as poor quality0
Sequence length51-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATCT278800.010.37473120
GTGTAGA396850.010.222165120
AGATCTC270800.010.149377120
GCGTCGG51650.09.875774120
GAGTGTA438300.09.679892120
AGTGTAG401300.09.585425120
TCTCGGT224450.09.544895120
AGAGTGT484150.09.457299120
GTAGATC384700.09.297081120
GTGGTCG141850.09.222522120
ATCTCGG242600.09.053424120
TCGGTGG198850.08.902643120
TGGTCGC125200.08.771408120
AAGAGTG548500.08.675991120
CTCGGTG214300.08.652843120
GATCTCG269650.08.6125765120
CGGTGGT201950.08.557979120
TGTAGAT425850.08.201417120
GGTCGCC127850.07.8855333120
TAGGGAA780650.07.856282120
Produced by FastQC (version 0.11.5)