FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830985

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830985
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11523913
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC130750.064.24132120
GAGCACA197650.043.15854120
AGAGCAC266150.033.18042120
AAGAGCA416550.021.310768118-119
GAAGAGC449900.019.614208120
GGAAGAG565850.016.103285120
TCGGAAG634350.014.5601635120
CGGAAGA647050.014.547142120
ATCGGAA761150.012.417256118-119
CACACGT33250.012.385168120
GATCGGA751000.012.28493116-117
AGATCGG837550.011.334955116-117
CACGTCT36800.011.190404120
TAGATCG212850.011.0248785120
CTAGATC53500.09.576421110-111
ACACGTC36200.09.285942118-119
AAGATCG351100.08.941752114-115
GAGATCG274550.08.320767116-117
TTAGATC95600.08.261422118-119
CAGATCG271100.07.8486767114-115
Produced by FastQC (version 0.11.5)