FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830986

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830986
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14191658
Sequences flagged as poor quality0
Sequence length58-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG46500.011.613197120
TAGATCT524150.09.879101120
GGTCGCC217150.09.643348120
TGGTCGC225650.09.599178120
GATCTCG473600.09.552606120
GTGGTCG246350.09.352783120
GGTGGTC287100.09.258332120
CTCGGTG360100.09.197678120
TCTCGGT391950.09.06261120
AGATCTC507450.08.962687120
ATCTCGG431750.08.8664665120
AGTGTAG714750.08.831283120
TCGGTGG351650.08.787368120
GTGTAGA686200.08.761516120
CGGTGGT347350.08.619765120
AGAGTGT837900.08.578806120
GAGTGTA769050.08.543223120
GTAGATC670600.08.321117120
GTCGCCG217000.08.129237120
AAGAGTG925500.07.8964715120
Produced by FastQC (version 0.11.5)