FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830987

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830987
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14191658
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC165150.072.74408120
GAGCACA254300.045.557774120
AGAGCAC339700.035.42468120
AAGAGCA530600.023.095795120
GAAGAGC588250.021.856329120
GGAAGAG754000.017.0162120
TCGGAAG869500.014.886708120
CGGAAGA894650.014.418156118-119
ATCGGAA1041400.012.538671120
GATCGGA1046000.012.440557118-119
AGATCGG1149400.011.21358116-117
TAGATCG251050.011.063681120
TTAGATC118100.09.917448120
CTAGATC64050.09.808605114-115
AAGATCG577700.09.214716114-115
CCTAGAT20300.08.572137120
GAGATCG352050.08.154777112-113
GCTAGAT25150.08.105088116-117
ACACGTC43750.08.02023118-119
CAGATCG331250.07.767847116-117
Produced by FastQC (version 0.11.5)