FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830989

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830989
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17610910
Sequences flagged as poor quality0
Sequence length50-126
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC286900.0115.83836120
GAGCACA487900.067.76621120
AGAGCAC654550.050.72576118-119
AAGAGCA962150.034.28902118-119
GAAGAGC1076850.030.649275116-117
CCGATCT82250.026.5889664
GGAAGAG1317450.024.956354116-117
TCCGATC94100.023.4630223
CGATCTA25400.022.8639035
CGGAAGA1521500.021.804457114-115
TCGGAAG1597400.020.551645114-115
ATCGGAA1875150.017.80245112-113
GATCGGA1910850.017.269808112-113
CTTCCGA139000.016.9276261
AGATCGG2065150.016.147154110-111
CACGTCT63650.016.109236120
ACACGTC63250.015.144592118-119
TAGATCG611300.014.985561112-113
CACACGT59450.014.977931118-119
ACGTCTG80300.013.777109120
Produced by FastQC (version 0.11.5)