FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830997

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830997
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11174145
Sequences flagged as poor quality0
Sequence length50-126
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC123300.071.762245120
GAGCACA199750.046.72957120
AGAGCAC263000.034.67017120
AAGAGCA419850.023.646938120
GAAGAGC447850.021.432444118-119
GGAAGAG575750.017.478336120
TCGGAAG694000.014.238791118-119
CGGAAGA706700.013.982909118-119
GATCGGA816050.012.22402120
ATCGGAA829900.011.907123116-117
AGATCGG882900.011.443706120
TAGATCG203350.010.461693118-119
ACACGTC48050.010.394287120
CACGTCT50400.09.909633120
CTAGATC50650.09.652037114-115
AAGATCG387600.09.281108120
CACACGT49450.09.204163118-119
TTAGATC86450.09.056271120
GAGATCG281800.08.69809114-115
CAGATCG261550.08.275554118-119
Produced by FastQC (version 0.11.5)