FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830999

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830999
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13249577
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC137300.071.92525120
GAGCACA219150.047.661144120
AGAGCAC294850.035.222416120
AAGAGCA464500.023.712687120
GAAGAGC512400.020.972616118-119
GGAAGAG638550.017.155952120
TCGGAAG747700.014.470009118-119
CGGAAGA759100.014.305145116-117
ATCGGAA870700.012.840387120
GATCGGA884300.012.627931120
AGATCGG974950.011.481045118-119
GCGCTAG5352.5673138E-411.141994120
TAGATCG246750.010.750344118-119
CTAGATC58500.09.850031120
CCTAGAT21950.09.354087120
TTAGATC96150.09.02394120
AAGATCG369350.08.724123118-119
CAGATCG356500.08.471875120
GAGATCG332200.08.104771116-117
ATAGATC127450.07.275587116-117
Produced by FastQC (version 0.11.5)