FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831001

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831001
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18454312
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC183550.064.784004120
GAGCACA287000.043.873642120
AGAGCAC371150.033.23822120
AAGAGCA591700.022.586437120
GAAGAGC666000.020.125652120
GGAAGAG817000.017.063177120
TCGGAAG960150.014.826096120
CGGAAGA959100.014.589719120
ATCGGAA1140050.012.492508118-119
GATCGGA1164200.012.373753120
AGATCGG1282700.011.368455120
TAGATCG333350.010.852907118-119
CTAGATC72800.09.264376112-113
GAGATCG597900.08.958412120
TTAGATC108950.08.714593120
CAGATCG535250.07.3646836116-117
ATAGATC182450.07.2856183118-119
AAGATCG371200.07.020716120
AGAGATC231800.06.850347118-119
CCTAGAT32000.06.6503983116-117
Produced by FastQC (version 0.11.5)