FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831002

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831002
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15399310
Sequences flagged as poor quality0
Sequence length60-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG78950.014.439031120
AAAAAAA859200.08.777637120
GTGGTCG499750.08.596008120
TCGGTGG654950.08.290429120
GGTGGTC551850.08.121498120
CGCCGTA385400.08.032941120
TGGTCGC460400.07.8581185120
GGTCGCC441250.07.83203120
CTCGGTG671250.07.6868916120
TCTCGGG33050.07.6245537120
GTCGCCG437850.07.591385120
ATCTCGG761650.07.5228863120
GATCTCG813950.07.481779120
TAGATCT886850.07.448584120
TCTCGGT706550.07.4346967118-119
AGATCTC853200.07.397781120
CGTATCA344350.07.300465120
GCCGTAT403450.07.2720313120
TCGCCGT423900.07.1762118-119
CGGTGGT625250.07.0915427118-119