FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831005

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831005
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12623944
Sequences flagged as poor quality0
Sequence length50-126
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC114550.064.64476120
GAGCACA185900.039.799362120
AGAGCAC243200.032.173405120
AAGAGCA387600.022.368309120
GAAGAGC440500.020.288126120
GGAAGAG522350.016.907993118-119
TCGGAAG614000.014.855458120
CGGAAGA624050.014.799559120
ATCGGAA727150.013.094543120
GATCGGA749250.013.039161120
AGATCGG849500.011.376748118-119
TAGATCG197950.010.660686120
CTAGATC47750.09.584328120
TTAGATC67850.09.461806120
CCTAGAT21350.08.484809118-119
GAGATCG424050.08.393944118-119
AAGATCG254650.07.91258120
AGAGATC147700.07.617199120
ATAGATC107800.07.3998327114-115
TGAGATC154150.07.257243120
Produced by FastQC (version 0.11.5)