FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831007

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831007
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15249529
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC221150.095.92084120
GAGCACA376250.057.01766120
AGAGCAC498050.042.787956118-119
AAGAGCA744950.028.485985120
GAAGAGC854100.025.951471120
GGAAGAG1034450.021.011518118-119
CGGAAGA1223350.017.751354120
TCGGAAG1268350.017.109835116-117
ATCGGAA1462200.014.883773116-117
GATCGGA1499300.014.541638114-115
AGATCGG1652900.013.185647114-115
TAGATCG377350.012.330466120
CTAGATC90850.011.93033120
GAGATCG587650.011.1586110-111
TTAGATC128350.011.103718114-115
CACGTCT66300.010.859723120
CAGATCG557500.010.848878112-113
TCCGATC79800.010.7097343
AAGATCG500100.010.456822114-115
CACACGT63450.010.371382120
Produced by FastQC (version 0.11.5)