FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831009

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831009
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12451082
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC132500.060.764294120
GAGCACA200800.041.523327120
AGAGCAC264300.031.867445120
AAGAGCA422000.020.174788120
GAAGAGC452350.019.051586120
GGAAGAG559500.016.276196120
TCGGAAG626600.014.355158118-119
CGGAAGA641350.014.006028120
GATCGGA757850.012.96176120
ATCGGAA752800.011.9757395120
CTAGATC52800.011.350102120
AGATCGG847200.011.207959118-119
TAGATCG192800.010.845358120
AAGATCG401700.09.084692118-119
TTAGATC87600.08.977375116-117
CACACGT32850.08.923249120
ACACGTC38450.08.3012905120
GAGATCG306750.07.955869118-119
CCTAGAT18900.07.39331118-119
CACGTCT38750.07.228407120
Produced by FastQC (version 0.11.5)