FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831011

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831011
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14185552
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC211150.0102.07152120
GAGCACA364300.060.06539120
AGAGCAC485250.044.78745118-119
AAGAGCA731250.029.664825120
GAAGAGC822350.026.512886116-117
GGAAGAG972150.022.334295116-117
CGGAAGA1119950.019.511257114-115
TCGGAAG1172350.018.47761114-115
ATCGGAA1357050.016.205774112-113
GATCGGA1395550.015.605268112-113
AGATCGG1521800.014.408202110-111
CCGATCT77550.013.7862914
TCCGATC80150.013.6725523
TAGATCG349650.013.15429110-111
CTAGATC69150.012.563918114-115
CGATCTA18550.012.3914995
GAGATCG612200.012.066246112-113
CACGTCT54300.011.981719120
CAGATCG553250.011.558402110-111
TTAGATC123800.011.5412245112-113
Produced by FastQC (version 0.11.5)