FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831020

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831020
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15400807
Sequences flagged as poor quality0
Sequence length53-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA452200.011.001939120
AAAAAAA1031450.010.775515120
GCGTCGG103000.010.371176120
GCCGTAT560050.010.287022120
GTCGCCG733500.09.858984120
CGCCGTA602550.09.820396120
GTGGTCG862650.09.809113120
TCGCCGT688050.09.725234120
TGGTCGC793050.09.708928120
GGTCGCC741250.09.634462120
GGTGGTC940350.09.426189120
CCGTATC542950.09.063569120
TCGGTGG1116450.08.976818120
TCATTAA373200.08.8443575120
CGGTGGT1044150.08.747763120
CATTAAA371950.08.486848120
CTCGGTG1194150.08.417849120
ATCATTA432350.08.411674120
GTATCAT462350.08.408931118-119
ATCTCGG1371950.08.276145120
Produced by FastQC (version 0.11.5)