FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831023

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831023
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13327909
Sequences flagged as poor quality0
Sequence length50-126
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC123950.065.06765120
GAGCACA194700.041.723087120
AGAGCAC261500.034.882988120
AAGAGCA437800.020.391514120
GAAGAGC455150.019.806152118-119
GGAAGAG563000.015.902909120
CGGAAGA641950.014.128881120
TCGGAAG649200.014.041002120
GATCGGA760950.012.089757120
ATCGGAA776300.011.870354118-119
AGATCGG833350.011.249472120
TAGATCG228750.011.09432118-119
AAGATCG354800.09.903917120
CTAGATC58000.09.352345118-119
CACACGT33600.08.875848120
TTAGATC109550.08.488274118-119
CAGATCG257550.08.382507120
GAGATCG247900.08.290372120
TAAGATC158100.07.381284120
ACACGTC39900.06.8783665118-119
Produced by FastQC (version 0.11.5)