FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831025

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831025
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10698334
Sequences flagged as poor quality0
Sequence length50-126
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC119950.063.85739120
GAGCACA175550.042.362423120
AGAGCAC231750.034.268337120
AAGAGCA355750.022.028837118-119
GAAGAGC402950.019.472878118-119
GGAAGAG500050.016.655546120
TCGGAAG569850.014.408288118-119
CGGAAGA583300.013.884926120
GATCGGA673950.012.396821120
ATCGGAA669750.012.229772120
AGATCGG760350.010.996772118-119
TAGATCG172050.010.977574118-119
CTAGATC45950.010.418518120
TTAGATC63250.09.538847120
CACACGT38100.09.466868120
CCTAGAT16750.09.396378116-117
AAGATCG320150.09.135871120
GCTAGAT18950.08.9977255120
CACGTCT40900.08.658427120
GAGATCG283500.08.396961120
Produced by FastQC (version 0.11.5)