FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831029

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831029
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14892658
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC188450.086.39953120
GAGCACA321100.050.418045120
AGAGCAC428900.038.069366118-119
AAGAGCA658950.024.70836118-119
GAAGAGC740700.022.222715120
GGAAGAG892950.018.93708120
CGGAAGA1023750.016.353374118-119
TCGGAAG1045300.015.883134118-119
ATCGGAA1213900.013.827015116-117
GATCGGA1252250.013.782775120
AGATCGG1370500.012.286092118-119
CACACGT47500.011.866573120
TAGATCG305200.011.688998120
CTAGATC93450.010.7651615118-119
GAGATCG517000.010.219161118-119
CAGATCG499100.09.757079114-115
GGTGTTT99500.09.7003371
AAGATCG405200.09.323464118-119
TTAGATC102550.09.3231125120
GGTGTAG76950.09.24220851
Produced by FastQC (version 0.11.5)