FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831030

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831030
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11297480
Sequences flagged as poor quality0
Sequence length54-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC56200.018.3620833
CCGATCT51100.018.3161244
AAAAAAA1661450.014.856236120
CTTCCGA98850.010.7468261
TCATTAA536700.09.971603120
CGATCTA14700.09.7954365
CATTAAA518750.09.773056120
CGTATCA727700.09.514483120
GTATCAT701650.09.405977120
CCGTATC823500.09.3912120
ATCATTA611450.09.360934120
CGCCGTA917300.09.032626120
TATCATT710150.08.887864120
GCGTCGG55600.08.740615120
GCCGTAT858800.08.732724120
TCGCCGT1014900.08.536419120
TGGTCGC1193000.08.529374120
TTCCGAT139050.08.500122
GGTCGCC1117100.08.421429120
GTCGCCG1073100.08.285902120
Produced by FastQC (version 0.11.5)