FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831031

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831031
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11297480
Sequences flagged as poor quality0
Sequence length50-126
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC180050.0104.77142120
GAGCACA294350.064.474434120
AGAGCAC396700.047.519753118-119
AAGAGCA578400.032.963093118-119
GAAGAGC642350.029.067848116-117
GGAAGAG787450.023.70051116-117
CGGAAGA920450.020.16699114-115
ACACGTC55400.019.936756120
CACGTCT51600.019.876041120
TCGGAAG939650.019.801561114-115
CACACGT52750.018.27962118-119
ATCGGAA1110650.017.097069112-113
GATCGGA1122050.016.927269112-113
AGATCGG1226700.015.566428110-111
TAGATCG264100.014.507162110-111
ACGTCTG66250.014.157664120
CGTCTGA74900.014.044076120
CTAGATC76250.013.855321110-111
GCACACG66350.013.806141118-119
GAGATCG360000.013.418951110-111
Produced by FastQC (version 0.11.5)